C34554 thermo
WebMar 16, 2024 · The medium was then removed and 5 × 10 4 CFSE (C34554, Thermo Fisher Scientific, Waltham, U.S.)-labeled HepG2 cells, which had previously been co-cultured with 1 × 10 5 N-SN or T-SN -pretreated neutrophils (at a 1:2 ratio) in the presence or absence of 10 U DNase I, were washed and suspended in 100 μl serum-free EC … WebDriving Directions to Tulsa, OK including road conditions, live traffic updates, and reviews of local businesses along the way.
C34554 thermo
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WebChlamydia pneumoniae is responsible for up to 20% of community acquired pneumonia and can exacerbate chronic inflammatory diseases. As the majority of infections are either mild or asymptomatic, a... WebCellTrace™ CFSE C34554, C34570 492/517 nm 488-nm CellTrace™ Yellow C34567, C34573 555/580 nm 532-nm or 561-nm CellTrace™ Far Red C34564, C34572 630/661 …
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WebFeb 17, 2024 · Splenocytes were isolated as previously described , seeded into 96-well U-bottom microtiter plates, stained with CFSE (C34554, Thermo Fisher Scientific) as per the manufacturer’s instructions, stimulated as previous described , and incubated for 72 h (5% CO 2, 37°C), the last 12 h of which was in the presence of Brefeldin A (00-4506-51 ... WebJan 24, 2024 · One hundred micrograms (µg) of pure exosomes were labeled with total protein stain CFSE (C34554, Thermo Fisher, Waltham, MA, USA) in 1 ml PBS according to the manufacturer’s instructions, washed by ultracentrifugation at 100,000 G for 3 h and resuspended in PBS. Three hundred µg of pure exosomes were labeled with DiR …
Web1.1.2药物与试剂 反式肉桂醛，购于上海源叶生物有限公司(批号H02M6Q1，检测纯度≥99%)；高糖DMEM细胞培养基、胎牛血清(FBS)、胰蛋白酶及青霉素-链霉素购于美国Thermo 公司；Cell Counting Kit-8(CCK-8试剂)购于日本同仁研究所(Dojindo)；1%结晶紫(批号V5265)、DMSO(批号D2650)购于 ...
WebMar 21, 2024 · The antibody-mediated blockade of co-inhibitory receptors PD-1 and CTLA-4 has been shown to clinically improve anti-tumor immune responses in multiple human malignancies 1. In contrast, agonising... fight riddleWebDocuments and Certificates. Search All. Order Status. Quick Order. Sign in. Error: This product (Catalog number 88954) has been discontinued and replaced by Catalog … fightright technologiesWebThermo Fisher c34554 mouse cd1d tetramer C34554 Mouse Cd1d Tetramer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 … grit song lyricsWebDeep red excitation/emission spectra (577/602nm maxima) are optimal for multiplexing with green fluorescent dyes and proteins. Easy to use: remove medium, add dye, incubate 30 minutes, and image cells. Fluorescent signal retention of >72 hours (typically three to six generations) Low cytotoxicity: does not affect viability or proliferation. fightrightWebIf required, the cells were labeled with Cell Trace CTV (C34557; Thermo Fisher) or CFSE (C34554; Thermo Fisher). Cells were re-suspended in pre-warmed assay medium (37°C) to obtain a cell density of 200,000 cells/mL. Then 100 µL of the cell suspension was transferred into a 96 U-bottom well plate, corresponding to 20,000 target cells per well grits priceWebApr 13, 2024 · Cells were reprogrammed using a CytoTune™-iPS 2.0 Sendai Reprogramming Kit (Thermo Fisher Scientific, Waltham, MA, USA) under feeder-free conditions, following the manufacturer’s instructions. ... Cat# C34554) and were co-cultured with irradiated (3000 cGy) stimulator myogenic progenitor cells (200,000 cells) in a 1-way … grits pronunciationfight right biel