TīmeklisEZRMI-13K, EMD Millipore, Billerica, MA, USA) and plasma triglycerides (Serum Triglyc-eride Determination Kit, Sigma TR0100, St. Louis, MO, USA) were measured as per the manufacturer’s instructions. 2.3. Tissue Collection and Histological Analysis Visceral fat pads in the perigonadal region (epididymal fat pads in males and pe- Tīmeklis2024. gada 22. dec. · Cultured pancreatic beta cells: Insulin secretion studies from BRIN-BD11 cells were conducted using a Millipore Rat/Mouse Insulin ELISA kit (cat no. EZRMI-13K) as described by Asalla et al., 2016. In brief, the cells were seeded in 24-multi-well plates at a density of 100,000 cells/well.

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Tīmeklislinked immunosorbent assay kits (EZMADP-60K and EZRMI-13K, respectively, Linco Research, St Charles, MO) according to manufacturer’s instructions. Plasma NEFA were measured using an enzymatic assay formatted for microtiter plates (ACS-ACOD MEHA, WAKO Chemicals USA, Richmond, VA). Measurement of Liver Lipids TīmeklisThis kit is used for the non-radioactive quantification of insulin in mouse and rat sera. Plasma samples may also be used but application to samples of other biological fluids may need validation by the user. Each kit is sufficient to run one 96-well plate including, in duplicates, background, 6 rat insulin standards, 2 quality controls and 39 ... da hood script superpowers

Rat/Mouse Insulin ELISA Kit - LINCO Research

Tīmeklis2024. gada 24. janv. · Thyrotropin (TSH) and free thyroxine (fT4) were measured with a sandwich ELISA kit from MyBiosource (San Diego, CA, USA, #MBS777023 and #MBS3805923) and insulin with a sandwich ELISA … Tīmeklis2024. gada 22. febr. · # EZRMI-13K). The protocol supplied by the kit manufacturer was followed. Briefly, a 96-well plate pre-coated with monoclonal mouse anti-rat insulin antibodies was loaded with six rat insulin standards, two quality controls, background, and samples. Each sample was diluted 2-fold with the assay buffer according to the … TīmeklisEZRMI-13K 96-well strip plate Recuperando preço... O preço não pôde ser recuperado Quantidade mínima necessária para ser múltipla de Maximum Quantity is Após … biofenac bcfi