Fastpfu polymerase是什么
WebApr 28, 2024 · The raw materials used for PCR reaction were as follows: Transgen AP221–02:Transstart FastPFU DNA Polymerase, 20μL reaction system: 5 × TransStart FastPfu buffer (4μL), 2.5 mM dNTPs (2μL), forward primer (5μM) 0.8μL, reverse primer (5μM) 0.8μL, TransStart FastPfu polymerase 0.4μL,BSA 0.2μL, template DNA 10 ng, … WebPfuDNA聚合酶（Pfu DNA polymerase），又称Pfu聚合酶，或Pfu酶，是在嗜热的古核生物火球菌属内发现的，一类能在活体内进行DNA复制的酶。体外实验中，在聚合酶链反 …
Fastpfu polymerase是什么
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WebCharacteristics. - TransStart® FastPfu Fly DNA Polymerase offers 108-fold fidelity as compared to EasyTaq® DNA Polymerase. - Extension rate is about 2-6 kb/min. - PCR products can be directly cloned into pEASY®-Blunt vectors. - Amplification of genomic DNA fragment up to 15 kb. - Amplification of plasmid DNA fragment up to 20 kb. Webwas 20 μL, 4 μL 5*FastPfu buer, 2 μL 2.5 mm dNTPs, 0.8 μL primer (5 μM), and 0.4 μL FastPfu polymerase. Briey, 10 ng DNA template. PCR products were recovered using a 2% agarose gel, puried ...
WebEppendorf N13462C移液器、Eppendorf 5430 R小型离心机、Eppendorf5424R高速台式冷冻离心机：德国Eppendorf公司；NanoDrop2000超微量分光光度计：美国Thermo Fisher Scientific公司；DYY-6C电泳仪：北京市六一仪器厂；ABI GeneAmp®9700型聚合酶链式反应（polymerase chain reaction，PCR）仪：美国 ... http://www.sonicebiotek.com/uploadfile/200941171319585.pdf
WebFastPfu FLY Sets Higher Standards of High-Fidelity DNA Polymerase Performance. Whereas Phusion and Q5 failed, FastPfu FLY succeeded easily at... 1-877-661-4711 … Web地址：北京市海淀区永泰庄北路1号中关村东升国际科学园4号楼. E-mail：[email protected]. 客服热线：+86-400 898 0321
WebPfu DNA Polymerase. 0.5-1μl. ddH2O. up to 50μl. 注意事项： 1、PCR 反应体系加样时，最后一步加入 Pfu DNA Ploymerase，整个过程宜在冰上操作。. 2、取 Pfu DNA …
WebJul 6, 2024 · 包括：HiPure Tissue DNA Mini Kit（美基生物），琼脂糖（西班牙Biowest），EB （上海阿拉丁生化科技有限公司），2×Power Taq PCR MasterMix（北京百泰克生物技术有限公司），TransStart FastPfu Fly DNA Polymerase（北京全式金生物技术有限公司），根据人类11号染色体的序列（NC ... peter tiffany mdWebMar 8, 2024 · For each sample, PCR reactions for bacteria were carried out in triplicate 20-μL reactions with 0.4 μL of each primer at 5 μmol⋅L −1, 10 ng template DNA, 2 μL dNTPs at 2.5 mmol⋅L −1, 0.4 μL FastPfu Polymerase (TransGen AP221-02: TransStart FastPfu DNA Polymerase; TransGen Biotech), 4 μL 5× FastPfu buffer, and certified DNA-free ... startech filip morisWeb侯 磊，薛 蓓，薛会英 (西藏农牧学院，西藏 林芝 860000) 土壤线虫作为土壤动物中的优势类群，是草地生态系统中的消费者，关系到草地生产力的增减[1]。 peter ticktin wikipediaWebTransStart® FastPfu DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity, >99% homogeneous measured by SDS-PAGE. Each batch of TransStart® FastPfu DNA Polymerase has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA. peter ticktin newsmaxhttp://www.shinegene.org.cn/tools/FastPfu.pdf startech firewire adapterWebFeb 24, 2024 · The PCR comprised a 20 μL reaction volume containing 4 μL of 5 × FastPfu buffer, 2 μL of 2.5 mmol/L dNTPs, 0.5 μL of 5 U/μL FastPfu polymerase, 0.5 μL of primer (5.0 μmol/L), and 10 ng DNA templates. PCR reaction conditions were as follows: 95°C for 3 min, 30 cycles at 95°C for 30 s, 57°C for 30 s, 72°C for 45 s, and 72°C for 10 min. startech finance share priceWeb合理选择耐热dna聚合酶是pcr成败与否的一个关键因素。选择最合适的耐热聚合酶，是进行pcr实验首先要考虑的问题。许多耐热dna聚合酶的主要区别在于特异性、保真性、耐热 … startech finance