Fathead minnow fhm cells
WebAug 1, 2024 · The general approach taken in these studies has involved exposing fathead minnows to a contaminant of interest, followed by some assessment of immunity, often immune-related gene expression or immune cell function, in the absence of in vivo immunological stimuli (e.g., pathogens, mitogens, etc.). While these studies provide … WebDec 8, 2015 · This study was conducted to investigate the protective effect of L‐carnitine (LC) against H 2 O 2 ‐induced oxidative stress in the fathead minnow muscle cell line (FHM). The FHM cells were stimulated with 1 mM H 2 O 2 for 1 h after LC pre‐treatment, and the cell viability and the activity and mRNA relative expression of antioxidant …
Fathead minnow fhm cells
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WebAug 23, 2016 · The present study utilizes the OECD 210 Early-life Stage (ELS) study to investigate the impacts levonorgestrel (LNG), a synthetic progestin, on fathead minnow (FHM) survival and growth.
WebThe fathead minnow testis, like the ovary, has bilateral elongate lobes located within the abdominal cavity and suspended from the gas bladder by mesenteries. ... Germ cells progress through six distinct cytological stage during spermatogenesis; primary spermatogonia, secondary spermatogonia, primary spermatocyte, secondary … WebABSTRACT: Invasion of Edwardsiella ictaluri into cultured mammalian, fish and enzymatically harvested catfish enteric epithelial cells is described. Gentamicin survival assays were used to demonstrate the ability of this catfish pathogen to invade IEC-6 (origin: rat small intestinal epithelium), Henle 407 (origin: human embryonic intestinal …
WebThe samples were inoculated on to cells of the fathead minnow cell line (FHM), incubated at 30 degrees C and observed for 10 days. Cytopathic effect was noted. Medium from cultures were then passaged onto a new cultures for a blind passage (on cultures that had no cytopathic effect) and to confirm infectivity (on cultures that showed cytopathic ... WebDec 16, 2006 · Two fish cell lines, grouper spleen (GS) (Qin et al., 2006) and fathead minnow (FHM) epithelial cells (Gravell and Malsberger, 1965), were grown in Leibovitz's …
WebThe fathead minnow Pimephales promelas Rafinesque is a member of the fish family Cyprinidae, the largest family of fish with more than 2,000 species worldwide and nearly …
WebINIS Repository Search provides online access to one of the world's largest collections on the peaceful uses of nuclear science and technology. The International Nuclear Information System is operated by the IAEA in collaboration with over 150 members. doylestown pavilionWebJul 1, 1993 · The xenobiotics were applied at different concentrations to cultured fathead minnow (FHM) fish cells. After 2 hr contact time the reduction of the total protein content was measured. The results are quantified by the PI 50 value, which is the concentration of test compound required to induce a 50% reduction in total protein content. doylestown pa travelWebAbstract. The viral susceptibility range of a poikilothermic cell line derived from the fathead minnow (Pimephales promelas) (FHM) to infection by a number of homoiothermic … cleaning red wine stains from carpetWebNov 6, 2024 · The fathead minnow (FHM, Pimephales promelas) is a member of the Cyprinidae family with a broad distribution in aquatic environments, both in running and still waters, ... Between 75 and 125 pM of library from each size fraction was loaded onto eight SMRT cells for PacBio RS II sequencing. cleaning red wine out of upholsteryWebMar 11, 2024 · In fish cells, there are a handful of carefully controlled experiments with convincing evidence of gene knockdown driven by shRNA-produced by a plasmid. Xie et al. provided convincing data on the fathead minnow (FHM) cell line at 29°C by using co-transfection experiment with a lacZ- and corresponding shRNA-expressing plasmids. … cleaning reels after saltwater fishingWebJul 3, 2024 · The cell line designated FHM, originating from tissue of the northern fathead minnow (Pimephales promelas) (Gravell and Malsberger 2010). Until lately, FHM cells … doylestown pa votingWeb（ Oncorhynchus mykiss） 性腺细胞系（ RTG － 2 ， 是被公认的第一株真骨鱼类的永生性细胞系 。随后 Gravell [4 ] Maisberger 1965 和 于 建立了另一株鱼类细胞系—胖头鲤（ Fathead minnow ） 肌肉细胞系（ FHM） 。鱼类 细胞系的建立表明鱼类体外培养技术的成 … doylestown pa university