WebThe cells preserved in our CM-ACF freeze medium can be used for cell counting, viability and cryopreservation, cell culture, mammalian cell culture, gene expression analysis … JEG-3 is a hypertriploid, clonally-derived, human cell line with epithelial morphology that was isolated from the Woods strain of the Erwin-Turner tumor. This cell line is tumorigenic, capable of forming malignant tumors consistent with choriocarcionoma. Genes expressed: human chorionic gonadotropin (hCG); human chorionic somatomammotropin ...

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WebWe measured the basal levels of phosphorylated ATR kinase in SW1353, CS-1, 5-8F, and JEG-3 cells. The results showed that the chondrosarcoma CS-1 cell line and SW1353 cells expressed very low levels of phosphorylated ATR at baseline. As experimental controls, 5-8F and JEG-3 expressed moderate levels of phosphorylated ATR . WebPeripheral blood mononuclear cells and placental tissue were collected. The human JEG3 trophoblast cell line was cultivated in 5.5 mmol/L or 30 mmol/L glucose and transfected with wild-type(wt)-p66 Shc and p66 Shc si RNA. P66 Shc and Drp1 m RNA levels were detected by quantitative real-time polymerase chain reaction. sibley warriors

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WebThe JEG-3 line cells were treated by 4 µmol CFSE, then 3 × 10 4 JEG-3 line cells in 50 µL of DMEM medium were inserted in 96-well plates with 10% FCS. NK-92 line cells in 100 … Web13 apr 2024 · To verify the function of miR-526b-5p on the trophoblast cells and explore the intrinsic molecular mechanism, transcriptome sequencing was performed on the HTR8 cells overexpressing miR-526b-5p. The miR-526b-5p overexpressing HTR8 cell line was used as the experimental group. The pCD513B-1 empty vector cell line was transfected as the … Web13 mar 2024 · The culture of JEG-3 cells (a human choriocarcinoma cell line) has been described previously . To study the cytotoxic effect of α-solanine on JEG-3 cells, cells were cultured in 96-well plates overnight, followed by treatment with different concentrations of α-solanine (10, 20, 30, 40 and 50 μM) or vehicle (0.5% v/v DMSO) for 24 h. sibley vs twc