WebCells were fixed with 4% PFA, permeabilized with 0.2% Triton X-100, and co-stained with Tubulin (66031-1-Ig; 1:100; red), under 40 x. IF Tip 2: Choose the right detergent to stain your target - Aldehyde fixation requires the cells to be permeabilized to allow antibodies access into the cells. WebMay 26, 2024 · For permeabilization, cells were treated with 0.1% Triton X-100 (v/v) for 5 min prior to blocking. After one remaining wash, the cells were incubated overnight at 4 °C with the rabbit monoclonal NOX4 (47-6) antibody at 2 …
Carboxylesterase 2a deletion provokes hepatic steatosis and …
WebTF-1 erythroblast cells were alcohol-fixed overnight, washed, and then suspended in 0.1% Triton X-100/PBS/1% BSA before staining with anti–histone H3[pS10] purified antibody complexed with Zenon Alexa Fluor 488 Rabbit IgG labeling reagent and FxCycle Violet stain. The pH3 signal (red) identifies cells that are in mitosis. WebNov 9, 2006 · Fluorochrome solution 0.1% sodium citrate (wt/v), 0.1% Triton X-100 (v/v), 50 mg l −1 PI in deionized/distilled water). Fluorochrome solution can be kept in the dark at 4 °C for months. products to help memory
Triton X-100 - an overview ScienceDirect Topics
WebFeb 26, 2008 · Prepare propidium iodide (PI)/Triton staining solution with RNAse A. Recipe: 10 ml of 0.1% (v/v) Triton X-100 (Sigma) of PBS (Triton is a viscous liquid), 2 mg DNAse-free RNAse A (Sigma), 200 microliters of 1 mg/ml PI (Molecular Probes). Prepare fresh! 2. Warm tubes to 37°C for 5-10 minutes (otherwise ice crystals can tear apart your … WebSep 21, 2024 · Then, the cells were washed at least once with cold phosphate-buffered saline (PBS), resuspended in 300–500 µL PI/Triton X-100 staining solution (10 mL 0.1% (v / v) Triton X-100 in PBS containing 2 mg DNAse-free RNAse A and 0.40 mL of 500 µg/mL PI), and incubated for 30 min at 20 °C. The fluorescence was measured using a … WebRed = Propidium Iodide (PI)/RNase Staining Solution #4087. Fixation and PermeabilizationConditions for Multiplexing. If you are multiplexing with antibodies that call for different CST protocols ... Confocal IF analysis of NIH/3T3 cells, permeabilized with 0.3%Triton ® X-100 (left) or methanol (right), using #3501 (green) ... relevance to the wider scientific audience