WebFig. 1. Dynamic mRNA and protein expression changes during ATII-to-ATI cell trans-differentiation in vitro. (A) Immunofluorescence staining of pmATII cells on coverslips for epithelial cell marker expression at day 2 after isolation. Fluorescent images represent a 400× magnification. The scale bar represents 50 µm. (B) mRNA expressions of epithelial … WebNotably, Nintedanib stabilized expression of distal lung epithelial cell markers, especially Surfactant Protein C in pmATII cells as well as in murine and human 3D-LTCs. Conclusions: Pirfenidone and Nintedanib exhibit distinct effects on murine and human epithelial cells, which might contribute to their anti-fibrotic action.

Regulation of Immunoproteasome Function in the Lung

Web0 Followers, 1 Following, 0 Posts - See Instagram photos and videos from @htt.pmatii WebpmATII cells were washed with ice-cold PBS and frozen at 280°C. After lysing cells with T-P er (Thermo Fisher Scientific) and centrifugation at 15,000 g for 30 min, supernatant was … the hippie trip yablonsky

Effect of in vitro treatment with Pirfenidone and Nintedanib on …

http://www.kjgbw.com/archives/44023.html Tumor-free lung tissue from lung cancer resections of patients without a co-morbidity of IPF/ILD was used to generate human 3D lung tissue cultures (3D … See more Pathogen-free female C57BL/6 mice (6–8 wk. old) purchased from Charles River were used in all studies. The mice were housed in rooms with constant humidity … See more The pmATII cells were isolated from mice as previously described [8]. The pmATII cells were seeded in 12 well-tissue culture plates and cultured in DMEM-F12 … See more Human and murine 3D-LTCs and 4 mm-punches thereof were generated as previously described [25, 28]. The amount of slices generated from one mouse … See more IF was performed as previously described [25, 28]. Briefly, 3D-LTCs were fixed with acetone/methanol (AppliChem, Germany) for 20 min or as indicated … See more Webwww.nature.comscientificreports SCIENTIFIC REPORTS 5:10230 DI: 10.1038srep10230 3 of LMP2 and LMP7 between these groups (Fig. 2c). Of note, expression of the 20S proteasome was also the hippie runner