Raw-blue cells
WebJan 21, 2024 · Raw-blue™ cells were cultured at 37 °C using manufacturer recommendations (InvivoGen) in a 24-well microplate until 70–80% confluence (1 × 10 5 cells/well) was reached. This cell line expressed the secreted embryonic alkaline phosphatase (SEAP) gene under the control of a promoter inducible by the transcription … WebRAW‑Dual™ cells stably express two reporter genes encoding SEAP (secreted embryonic alkaline phosphatase) and Lucia luciferase . SEAP expression depends on the activation of the endogenous MIP-2 promoter. MIP‑2, also known as CXCL2, is the murine homolog of human IL-8, a chemokine produced in an NF-κB-dependent manner [3].
Raw-blue cells
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WebJun 30, 2011 · During the course of blocking CD36 oxLDL binding function using anti CD36 antibodies, we have identified a novel domain of CD36 that triggers inflammatory response-independent of oxLDL binding. OxLDL bound to the mouse reporter cell line RAW-Blue induced TNF-α and RANTES mRNA and protein expression. Pretreatment of RAW-Blue … WebJun 28, 2024 · QUANTI-Blue colorimetric enzyme assay: RAW-Blue cells were added at 1 × 10 5 cells per well in a 96-well plate and sequentially the same amounts of different particles at a particle: cell ratio ...
WebAug 24, 2010 · RAW-Blue cells are derived from RAW-264.7 macrophages that stably express a secreted embroyonic alkaline phosphotase (SEAP) gene which can be induced by NF-κB or AP-1 transcription factors. Treatment of RAW-Blue cells with Rx inhibited the LPS-induced NF-κB activity in a time- and dose-dependent manner as indicated by the reduced … WebRAW 264.7 cells are a macrophage-like, Abelson leukemia virus-transformed cell line derived from BALB/c mice. This cell line is a commonly used model of mouse macrophages for the study of cellular responses to microbes and their products. InvivoGen has developed RAW-Blue ™ cells and RAW-Lucia ™ cells, which express a secreted inducible ...
WebRAW-Blue™ cells were derived from the murine RAW 264.7 macrophages with chromosomal integration of a SEAP reporter construct inducible by NF-κB. RAW-Blue™ cells express many pattern-recognition receptors (PRRs), including all toll-like receptors (TLRs) except for TLR5, but with TLR4 and TLR8 expressed at the highest levels. WebRAW 264.7. TIB-71 ™. RAW 264.7 is a macrophage cell line that was established from a tumor in a male mouse induced with the Abelson murine leukemia virus. Product category.
WebThe in vitro model using RAW 264.7 cells has been widely used. However, there is still no consensus on which inflammatory mediators should initially be measured to screen for possible anti-inflammatory effects. To determine the rationality of measuring inflammatory mediators together with NO, such as the levels of tumor necrosis factor ...
WebFeb 13, 2016 · Anti-inflammatory bioassay. RAW-Blue cells (5 × 10 4 /well) were cultured in 96-well plates for 24 h, and then treated with LPS (1 μg/mL) alone or together with test samples for 20 h. Secreted embryonic alkaline phosphatase (SEAP) activity in the medium was determined by QUANTI-Blue medium following the manufacturer’s instructions. how do i evolve my budewWebTherefore, RAW-Blue™ cells can be used as: TLR reporter cells: they express all TLRs, with the exception of TLR5, at different levels NOD reporter cells: … how do i evolve cosmog in pokemon shieldWebSep 5, 2024 · RAW-Blue cells and HEK 293 h-TLR transfected cells Murine RAW-Blue, HEK-BLUE-hTLR2 and HEK-BLUE-hTLR4 cells carrying a SEAP reporter construct were purchased from InvivoGen (San Diego, CA, USA). how much is rent subsidyWebCo-immunoprecipitation experiments using cell lysates from RAW-blue cells further dem- onstrated that Neu1 forms a complex with TLR9 receptors in naive and ODN treatment of cells for 30 min (Fig. 4C). how do i evolve munchlaxWebLipofectamine 3000 reagent is a specially formulated gentle transfection reagent that is optimized for plasmid delivery into CHO cells with high efficiency and low cell toxicity. The reagent allows for transfection to be performed both in presence or absence of serum. The easy protocol, exceptional plasmid DNA delivery, and the ability to ... how much is rent insuranceWebRAW-blue cells were pretreated with 200μM Tamiflu, 100μg/mL MMP9i, or 100μg/mL BIM-23127 for 30min followed by 10μg/mL imiquimod or left cultured in media. Cells (1×107 cells) are pelleted ... how much is rent in victoria bcWebNF-κB activity from LPS-activated RAW-Blue™ cells after incubation with 30 and 300 μg/ml of acacia (AG), 30 and 300 μg/ml of guar gum (GG) and 30 μg/ml of 5ASA for 24 h in serum-free medium ... how do i evolve piloswine